Even then, you won’t be able to do that. CRISPR is a precision technique and you need exactly the right sequence and enzyme to make the alterations you want. You can’t get that in a starter kit.
You also can’t random bullshit go! your way with UV cause it will straight up kill bacteria and the ones that don’t die will just be more resistant to UV damage.
I was being very general and oversimplifying, but I think you may have misunderstood my post. I did a poor job explaining, so that’s not on you, but I did make it fairly explicit that for most people I had assumed they WERENT using crispr, just the kit.
As for the UV resistance, disclaimer: I haven’t ever done this. I am not speaking from experience, nor do I have the prerequisite information to confidently argue I am correct here, but I do believe you are slightly mistaken. I would love for you to correct me, especially if you actually do know what you’re talking about, unlike me.
this seems to suggest that UVC can induce mutations so long as you include a repair agent. From what I can gather, the UV damages the cell in such a way that would kill it normally, but by repairing the cell wall you can increase the chances the cell will heal with mutated DNA.
this seems to suggest that you can use UV to enhance or diminish existing traits in a bacteria, which is pretty much exactly what I was referring to.
this even seems to suggest that UV radiation induces a faster mutation rate than bacteria that naturally mutate quickly. This effectively eliminates the possibility of something similar to a placebo, showing that UV is more effective than otherwise naturally waiting for mutations.
Again, I could be mistaken, and there’s a bunch of specifics in those studies I didn’t quite understand. But I’m confident I got the general idea, and as such it seems a fairly difficult task to show that my original assessment was incorrect. This is going to sound facetious but I promise it’s not, I just value my word choice: I genuinely appreciate your attempt to correct me, and if you have any other information you feel would be beneficial to the discussion please don’t hesitate to share it with me.
Yes, you’re mistaken. That’s what I’ve been trying to tell you.
None of these studies are done with a DIY set, they’re done at million-dollar laboratories by professionals. Two of these studies found exactly what I told you in the previous comment, that the bacteria became better equipped to deal with UV damage.
The second study is interesting, as they found a way to select the bacteria to produce the chemical of interest. But you won’t be able to do this with a DIY kit either.
And lastly, how are you going to determine whether you’re even on the right track? Do you have a whole bunch of rhesus monkeys that you can inoculate to see whether they die and infect each other?
If I’m breeding for something like infectivity, then I set my petri dish with agar and growth medium inside a sealed box with other sterilized and preset, but not innoculated(I think that’s the right word), petri dishes. I induce a light draft using something like a fan. I also loaded a fair amount of self harvested human cells into each dish. I allow them to incubate after exposure to UV and if any survived and spread to a different dish to infect the cells there, I select the one that infected furthest away. I use a variety of different setups with different cells to ensure they have a general effect and don’t, say, only effect skin cells.
If I’m breeding for lethality I directly measure how much of a toxin they generate, and breed the ones that generate more.
If I’m breeding to induce new symptoms, then yes. I go around town infecting random people and stalking them to see what happens. If I’m doing biological terrorism I’m just guessing my own safety isn’t much of a concern.
As for the resistance to UV, I never denied that you would end up breeding for UV resistance. But pumped UV lasers are a thing, I’m guessing there will always be a sweet spot between a resistance they can’t overcome and simply vaporizing the bacteria. While an unintentional side effect, it’s not the only resistance you’d be breeding for either. Once you have your bacteria you’d go through rounds of exposing it the antibiotics and harvesting the survivors. Hell, that’s not a terrible way to make a super plague on its own.
The DIY kit is only valuable for the tooling, so I am glad we’re not stuck on that.
As for the million dollar lab vs home setup, I’m not convinced that it can’t be replicated. I highly doubt The Thought Emporium has a million dollar set up, and yet they are able to do actual gene editing and growing a rat brain on a computer chip(again, simplification). Absolutely no clue how accurate this is, but they’re only pulling in about $3000 a month from videos at the max. And they make $1300 a month from patreon.. And this is their intro into DIY lab setups. It does not list THEIR costs, but it gives a good idea of costs for a starting setup.
I’m… not done with this comment, but I’m currently at work and having to jump between multiple things is becoming difficult, so I’ll do more research tonight to present a better structured, funded, realistic, and deadly example of what a DIY setup can do. I think we’ve somewhat veered off my original topic, but I’ve really enjoyed this discussion and hypothetical, even though it mostly feels like a headstrong devils advocate situation at this point. I do have ADHD and my memory kinda sucks, so if I forget and you are interested in continuing then please message me sometime tomorrow either on here or in a dm
Even then, you won’t be able to do that. CRISPR is a precision technique and you need exactly the right sequence and enzyme to make the alterations you want. You can’t get that in a starter kit.
You also can’t random bullshit go! your way with UV cause it will straight up kill bacteria and the ones that don’t die will just be more resistant to UV damage.
Stop watching the crazy stuff :)
I was being very general and oversimplifying, but I think you may have misunderstood my post. I did a poor job explaining, so that’s not on you, but I did make it fairly explicit that for most people I had assumed they WERENT using crispr, just the kit.
As for the UV resistance, disclaimer: I haven’t ever done this. I am not speaking from experience, nor do I have the prerequisite information to confidently argue I am correct here, but I do believe you are slightly mistaken. I would love for you to correct me, especially if you actually do know what you’re talking about, unlike me.
this seems to suggest that UVC can induce mutations so long as you include a repair agent. From what I can gather, the UV damages the cell in such a way that would kill it normally, but by repairing the cell wall you can increase the chances the cell will heal with mutated DNA.
this seems to suggest that you can use UV to enhance or diminish existing traits in a bacteria, which is pretty much exactly what I was referring to.
this even seems to suggest that UV radiation induces a faster mutation rate than bacteria that naturally mutate quickly. This effectively eliminates the possibility of something similar to a placebo, showing that UV is more effective than otherwise naturally waiting for mutations.
Again, I could be mistaken, and there’s a bunch of specifics in those studies I didn’t quite understand. But I’m confident I got the general idea, and as such it seems a fairly difficult task to show that my original assessment was incorrect. This is going to sound facetious but I promise it’s not, I just value my word choice: I genuinely appreciate your attempt to correct me, and if you have any other information you feel would be beneficial to the discussion please don’t hesitate to share it with me.
Yes, you’re mistaken. That’s what I’ve been trying to tell you.
None of these studies are done with a DIY set, they’re done at million-dollar laboratories by professionals. Two of these studies found exactly what I told you in the previous comment, that the bacteria became better equipped to deal with UV damage.
The second study is interesting, as they found a way to select the bacteria to produce the chemical of interest. But you won’t be able to do this with a DIY kit either.
And lastly, how are you going to determine whether you’re even on the right track? Do you have a whole bunch of rhesus monkeys that you can inoculate to see whether they die and infect each other?
If I’m breeding for something like infectivity, then I set my petri dish with agar and growth medium inside a sealed box with other sterilized and preset, but not innoculated(I think that’s the right word), petri dishes. I induce a light draft using something like a fan. I also loaded a fair amount of self harvested human cells into each dish. I allow them to incubate after exposure to UV and if any survived and spread to a different dish to infect the cells there, I select the one that infected furthest away. I use a variety of different setups with different cells to ensure they have a general effect and don’t, say, only effect skin cells.
If I’m breeding for lethality I directly measure how much of a toxin they generate, and breed the ones that generate more.
If I’m breeding to induce new symptoms, then yes. I go around town infecting random people and stalking them to see what happens. If I’m doing biological terrorism I’m just guessing my own safety isn’t much of a concern.
As for the resistance to UV, I never denied that you would end up breeding for UV resistance. But pumped UV lasers are a thing, I’m guessing there will always be a sweet spot between a resistance they can’t overcome and simply vaporizing the bacteria. While an unintentional side effect, it’s not the only resistance you’d be breeding for either. Once you have your bacteria you’d go through rounds of exposing it the antibiotics and harvesting the survivors. Hell, that’s not a terrible way to make a super plague on its own.
The DIY kit is only valuable for the tooling, so I am glad we’re not stuck on that.
As for the million dollar lab vs home setup, I’m not convinced that it can’t be replicated. I highly doubt The Thought Emporium has a million dollar set up, and yet they are able to do actual gene editing and growing a rat brain on a computer chip(again, simplification). Absolutely no clue how accurate this is, but they’re only pulling in about $3000 a month from videos at the max. And they make $1300 a month from patreon.. And this is their intro into DIY lab setups. It does not list THEIR costs, but it gives a good idea of costs for a starting setup.
I’m… not done with this comment, but I’m currently at work and having to jump between multiple things is becoming difficult, so I’ll do more research tonight to present a better structured, funded, realistic, and deadly example of what a DIY setup can do. I think we’ve somewhat veered off my original topic, but I’ve really enjoyed this discussion and hypothetical, even though it mostly feels like a headstrong devils advocate situation at this point. I do have ADHD and my memory kinda sucks, so if I forget and you are interested in continuing then please message me sometime tomorrow either on here or in a dm